Trem1 Rat Monoclonal Antibody [Clone ID: L5-B8]

Specifications

Product Data
Clone Name	L5-B8
Applications	FN, IF, WB
Recommend Dilution	Flow cytometry: The typical starting working dilution is 1:50. Functional assays. Immunoflourescence. Western blot: The typical starting working dilution is 1:50. Not suitable for Immunohistochemistry on paraffin sections.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Mouse TREM-1-human IgG-Fc fusion protein
Specificity	The monoclonal antibody L5-B8 recognizes mouse triggering receptor expressed on myeloid cells-1 (TREM-1). TREM-1 is a 30 kDa monomeric transmembrane activating receptor.
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% bovine serum albumin
Concentration	lot specific
Purification	Protein G
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C.
Gene Name	triggering receptor expressed on myeloid cells 1
Database Link	Entrez Gene 58217 Mouse UniProt ID Q9JKE2
Background	TREM-1 is a 30 kDa monomeric transmembrane activating receptor. TREM-1 is a member of the immunoglobulin superfamily. TREM-1 is expressed at low levels in the early development of the hematopoietic system in the promonoistic stage, and at high levels on the surface of immune cells, including neutrophils, monocytes and macrofages. TREM-1 is synthesized as a 234 amino acid (aa) precursor with a signal peptide (16 aa), an extracellular domain (184 aa), a transmembrane domain (29 aa), and a short cytoplasmic domain (5 aa). The short intracellular domain associates with a signal-transduction molecule, DNAX-activation protein 12 (DAP12), triggering the secretion of inflammatory cytokines that amplify the host response to microbial agents. TREM-1 acts in synergy with Toll-like receptor signaling pathways in amplifying the inflammatory response. Platelets express a natural ligand for TREM-1. The expression of TREM-1 is greatly upregulated on phagocytic cells in the presence of bacteria and fungi. TREM-1 has a role in sepsis, inflammatory bowel disease (IBD) and multiple sclerosis. In contrast, TREM-1 is not upregulated in samples from patients with non-inefctious inflammatory conditions. During infections, receptor expression is modulated and soluble TREM-1 (sTREM-1, 17 kDa) is released. TREM-1 is shed from the membrane of activated phagocytes and can be found as sTREM-<1 in> body fluids like plasma and bronchoalveolar lavage fluid (BAL). Elavated levels of sTREM-1 have a accuracy and sensitivity in detecting microbial infections as underlying disease. Furthermore, sTREM-1 has been associated with non-infectious inflammatory conditions like major abdominal surgery, peptic ulcer disease and COPD.
Synonyms	TREM-1
Reference Data

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