TNF alpha (TNF) Mouse Monoclonal Antibody [Clone ID: 52B83]
CAT#: AM26298FC-N
TNF alpha (TNF) mouse monoclonal antibody, clone 52B83, FITC
Conjugation: Unconjugated
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CNY 6,000.00
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Specifications
Product Data | |
Clone Name | 52B83 |
Applications | IHC, WB |
Recommend Dilution | Immunohistochemistry on frozen sections (4): 6-µm tissue sections were fixed in acetone for 10 minutes (Ref. 4). Immunohistochemistry on paraffin sections (5): Tissue sections were fixed in 5% formalin embedded in paraffin and cut into 2µm sections (Ref. 5). Flow cytometry (3): Antibody 52B83 was used to stain soluble TNF bound to TNF receptors. Cells were fixed in PBA containing 0.2% formaldehyde (Ref. 3). Western blot: A reduced sample treatment and 15% SDS-Page was used. The band size observed is 17 kDa (Ref.4). The typical starting working dilution is 1:50. |
Reactivity | Guinea Pig, Human, Mouse, Monkey, Rhesus Monkey |
Host | Mouse |
Clonality | Monoclonal |
Specificity | The monoclonal antibody 52B83 reacts with tumor necrosis factor alpha (TNF-alpha). |
Formulation | PBS Label: FITC State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 1% bovine serum albumin Preservative: 0.02% sodium azide |
Concentration | lot specific |
Purification | Protein G |
Conjugation | FITC |
Storage Condition | Store at 2 - 8 °C. |
Gene Name | tumor necrosis factor |
Database Link | |
Background | TNF-alpha is a homotrimeric 17 kDa protein, that interacts with either one of the two types of TNF-receptors, termed I and II, leading to receptor cross-linking and signal transduction. The receptors differ strongly in their intra-cellular signaling pathways. |
Synonyms | TNF, TNF-a, TNFA, TNFSF2, Cachectin |
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