IP10 (CXCL10) Mouse Monoclonal Antibody [Clone ID: 6D4]

Specifications

Product Data
Clone Name	6D4
Applications	ELISA, IF, IHC, WB
Recommend Dilution	Immunohistochemistry on Frozen Sections (4): Tissue sections were fixed 10 minutes in acetone and pretreated with avidin/biotin blocking kit. As Positive Control chronically inflamed liver was used and as Negative Control normal liver and isotype matched antibody (Ref.4). The typical starting working dilution is 1/50. Flow Cytometry (3): Antibody 6D4 stains human CXCL10. For intracellular staining activated plasmacytoid dendritic cells were fixed with 2% paraformaldehyde and permeabilized with 0.5% saponin. All steps were performed in presence of 0.5 M EDTA to avoid cell aggregation (Ref.3). The typical starting working dilution is 1/50. Immunoassays (1,2). Immunoflourescence (4). Western blot (4): Tissue was homogenized in loading sample buffer and subjected to SDS-PAGE. The band size is ~9 kDa (Ref.4). The typical starting working dilution is 1/50. Positive Control: Acitvated plasmacytoid dendritic cells. Negative Control: Normal liver.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human IP-10
Specificity	The monoclonal antibody 6D4 recognizes Human C-X-C motif chemokine 10 (IP-10), a protein of 98 amino acids.
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Gene Name	C-X-C motif chemokine ligand 10
Database Link	Entrez Gene 3627 Human UniProt ID P02778
Background	IP-10, also known as CXCL10, functions as ligand for the CXCR3 receptor. IP-10 belongs to the α-chemokine (C-X-C) family, which can be divided in two subfamilies: (1) potent chemoattractants for neutrophils, like IL-8 and (2) potent chemoattractants for lymphocytes, like the IFN gamma inducible protein (IP)-10. IP-10 is produced by a wide variety of cell types ranging from neutrophils, dendritic cells and monocytes to hepatocytes, endothelial cells and keratinocytes. The cytokine is reported to be involved in a scala of inflammatory pathologies such as HIV, encephalitis, cutaneous T cell lymphoma, chronic hepatitis, psoriasis and acute anterior uveitis. Various observations strongly suggest a role for the C-X-C chemokines IL-8 and IP-10 in the regulation of angiogenic activity in cancer and in idiopathic pulmonary fibrosis. Furthermore IP-10 is associated with acute rejection processes estimated by the predictive properties of urinary IP-10 expression for the short- and long-term graft function after kidney transplantation.
Synonyms	C-X-C motif chemokine 10, Small-inducible cytokine B10, Gamma-IP10, INP10, SCYB10
Reference Data

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