PR3 (PRTN3) Mouse Monoclonal Antibody [Clone ID: PR3-G2]
CAT#: AM26220FC-N
PR3 (PRTN3) mouse monoclonal antibody, clone PR3-G2, FITC
Conjugation: Unconjugated
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CNY 6,656.00
货期*
5周
规格
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Specifications
Product Data | |
Clone Name | PR3-G2 |
Applications | ELISA, IHC, WB |
Recommend Dilution | Immunohistochemistry on frozen section: The typical starting working dilution is 1:50. Flow cytometry: The typical starting working dilution is 1:50. Immunoassay. Western blot: The typical starting working dilution is 1:50. |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | A crude granule extract |
Specificity | Monoclonal antibody PR3G-2 reacts with human proteinase 3 (PR3), a 30 kDa protein. |
Formulation | PBS Label: FITC State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 1% bovine serum albumin Preservative: 0.02% sodium azide |
Concentration | lot specific |
Purification | Protein G |
Conjugation | FITC |
Storage Condition | Store at 2 - 8 °C. |
Gene Name | proteinase 3 |
Database Link | |
Background | PR3 is a major antigen recognized by autoantibodies directed against cytoplasmic proteins of neutrophilic granulocytes and monocytes (called anti-neutrophil cytoplasmic autoantibodies (ANCA)). ANCA are able to activate primed neutrophils to produce oxygen radicals and release lytic enzymes, including PR3. Proteinase 3 (PR3) was identified as the target antigen of ANCA in Wegener's granulomatosis (WG). ANCA directed against PR3 (PR3-ANCA) can interfere with the binding of PR3 to its physiological inhibitor alpha1-antitrypsin (alpha1-AT) and with the proteolytic activity of PR3. At the site of inflammation, PR3 can cleave the PR3-ANCA complex between these inhibiting ANCA and PR3 itself, leaving active PR3. Autoantibodies to PR3 are potent activators of the 5-lipoxygenase pathway in primed human neutrophils. Extracellular free arachidonic acid, as present at an inflammatory focus, synergizes with such autoantibodies to evoke full-blown lipid mediator generation, granule secretion and respiratory burst. Proteinase 3 (PR3) is a neutral serine proteinase, which is localized in the azurophilic granules of neutrophils and in granules of monocytes and can be detected in the membrane of secretory vesicles. PR3 degrades a number of extracellular matrix proteins such as elastin and inactivates human C1 inhibitor. Membrane-associated PR3 is also able to activate caspase-3 without triggering apoptosis of neutrophils, which is possibly a neutrophil survival mechanism. In addition, PR3 is involved in myeloid differentiation and is, therefore, also called myeloblastin. |
Synonyms | Myeloblastin, MBN, Leukocyte proteinase 3, Proteinase 3, PRTN3, PR-3, Neutrophil proteinase 4, NP-4, P29, Wegener autoantigen, AGP7 |
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