DC SIGN (CD209) Mouse Monoclonal Antibody [Clone ID: DCN47.5]

Specifications

Product Data
Clone Name	DCN47.5
Applications	FC, FN
Recommend Dilution	Flow cytometry: The typical starting working dilution is 1:50. Functional assay: Inhibits binding of DC-SIGN to ICAM-2 on endothelial cells.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	This antibody reacts with the C-type lectin, DC-SIGN (CD209), exclusively expressed on human dendritic cells (DC).
Formulation	PBS Label: FITC State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 1% bovine serum albumin Preservative: 0.02% sodium azide
Concentration	lot specific
Purification	Protein G
Conjugation	FITC
Storage Condition	Store at 2 - 8 °C.
Gene Name	CD209 molecule
Database Link	Entrez Gene 30835 Human UniProt ID Q9NNX6
Background	DC are specialized antigen presenting cells and bridge the innate and the adaptive immune system. They provide high levels of costimulation necessary for activation of both naïve and antigen-experienced T-cells. Immature DC are capable to migrate to inflammatory sites, capture antigen and process it internally to form MHC-peptide complexes. Following antigen uptake, DC undergo maturation and migrate to lymphoid organs where they can present MHC-peptide complexes to resting T-cells and drive T-cell proliferation. During differentiation and maturation of DC, several phenotypic surface markers are expressed: CD1a, CD4, CD11, CD40, CD86, and HLA-DR. Immature DC predominantly express CCR5 which enables DC to migrate to inflammatory sites, whereas mature DC express high levels of CXCR4, a receptor that facilitates migration to lymphoid organs.DC also express DC-specific, ICAM-3 grabbing, nonintegrin (DC-SIGN), a 44 kDa C-type lectin that binds to the HIV-1 envelope glycoprotein gp120, ICAM-3 on T-cells and ICAM-2 on endothelial cells. HIV virions are able to infect cells expressing CD4 and the chemokine co-receptors CCR5 or CXCR4 and can attach to DC-SIGN to extend virion lifespan. Therefore, DC are candidates for HIV-1 infection. DC-SIGN-ICAM-3 binding is integrin-independent but calcium-dependent and antibodies reactive against DC-SIGN can be used to study DC-SIGN-ICAM3 binding.
Synonyms	DCSIGN1, DCSIGN, DC-SIGN1, CLEC4L, Dendritic Cell Marker
Reference Data

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