Nitrotyrosine Mouse Monoclonal Antibody [Clone ID: EM-30]

Specifications

Product Data
Clone Name	EM-30
Applications	IHC, WB
Recommend Dilution	Western Blot.  Positive Control: NO2-BSA, NO2-HSA.  Immunohistochemistry on Paraffin Sections: 10 μg/ml Positive tissue: Colon cancer epithelium, prostate hyperplasia.  Immunohistochemistry on Frozen Sections: 10 μg/ml Positive tissue: Colon cancer epithelium, prostate hyperplasia.
Reactivity	All Species
Host	Mouse
Clonality	Monoclonal
Immunogen	NO2-Tyr-CH2-Thyroglobulin
Specificity	This antibody detects Nitrotyrosine group in biological material. It can be used as a marker for peroxynitrite formation in particular tissues.
Formulation	Phosphate buffered saline (PBS), pH~7.4 State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Nitrotyrosine can be detected in proteins from a variety of tissues usually in association with pathological conditions. Reaction of nitric oxide with superoxide produces peroxynitrite, which can undergo heterolytic cleavage into nitronium and hydroxyl ions. Nitration of tyrosine residues by nitronium ion forms nitrotyrosine groups in the respective proteins. Nitrotyrosine is thus a marker for inflammation-associated tissue damage.
Synonyms	NO-Tyrosine, Nitro-Tyrosine
Reference Data

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