pan Actin (pan alpha / gamma 2) Mouse Monoclonal Antibody [Clone ID: HHF35]

Specifications

Product Data
Clone Name	HHF35
Applications	IHC, WB
Recommend Dilution	Western Blot (Reducing conditions): 1 μg/ml. Positive Control: Murine femoral muscle, murine heart. Negative Control: HUVEC line. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: Antigen retrieval steps generally not required, but e.g. in case of arterial smooth muscle cells or myoepithelial cells, pepsin or trypsin pretreatment is recommended.
Reactivity	Canine, Chicken, Feline, Human, Mouse, Primate, Rabbit, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	SDS extracted protein fraction of Human myocardium.
Specificity	This antibody recognizes muscle-specific alpha and gamma actin (42 kDa) in various species. It stains skeletal, smooth and myocardial cells as well as myoepithelial cells and pericytes of small vessels. It is a widely used marker of muscle and muscle-derived cells. Cellular Localization: Cytoplasmic.
Formulation	PBS, pH~7.4 State: Aff - Purified State: Liquid purified Ig fraction (> 95% by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Actin is a highly conserved ubiquitous globular protein (G-actin) that polymerizes to form fibrous F-actin microfilaments. In higher eucaryotes several actin isoforms have been identified, that fall into three classes. Alpha actin is a structural component of the contractile apparatus of muscle cells or muscle-derived cells. Beta actin and gamma actin play roles in regulation of cell motility in other cell types. Specific subcellular structures such as as stress fibers, focal adhesions, filopodia etc., are formed by involvement of actin cytoskeleton.
Reference Data

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