Prion protein PrP (PRNP) Mouse Monoclonal Antibody [Clone ID: EM-20]

Specifications

Product Data
Clone Name	EM-20
Applications	IHC, WB
Recommend Dilution	Western blot: 0.5 μg/ml (Non-reducing conditions are essential). This clone has been described to work in Immunohistochemistry on Paraffin Sections (10 µg/ml).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human prion protein
Specificity	The Mouse monoclonal antibody EM-20 recognizes Human prion protein (PrP). Diglycosylated form of PrP has ~40 kDa, monoglycosylated form ~30 kDa, and nonglycosylated form ~19-21 kDa. This antibody is suitable for discrimination between normal cellular prion protein (PrPc) and its conformationally changed form (PrPSc) prion protein.
Formulation	PBS, pH~7.4 State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Preservative: 15mM Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	prion protein
Database Link	Entrez Gene 5621 Human UniProt ID F7VJQ1
Background	The mouse monoclonal antibody EM-20 recognizes human prion protein (PrP). Diglycosylated form of PrP has ~ 40 kDa, monoglycosylated form ~ 30 kDa, and nonglycosylated form ~ 19-21 kDa. This antibody is suitable for discrimination between normal cellular prion protein (PrPc) and its conformationally changed form (PrPSc) prion protein.
Synonyms	Major prion protein, PrP27-30, PrP33-35C, ASCR, PRNP, PRIP
Reference Data
Protein Families	ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transmembrane
Protein Pathways	Prion diseases

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