beta Actin (ACTB) Mouse Monoclonal Antibody [Clone ID: AC-15]

Specifications

Product Data
Clone Name	AC-15
Applications	IHC, IP, WB
Recommend Dilution	Western Blot: 0.25-0.5 μg/ml. Immunohistochemistry on Paraffin Sections: 0.5-1 μg/ml.
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
Specificity	This antibody reacts to Actin beta.
Formulation	PBS State: Purified State: Liquid purified Ig fraction Preservative: 0.02% Sodium Azide
Reconstitution Method	Restore with 1.2% sodium acetate or neutral PBS.
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	41.7 kDa
Gene Name	actin, beta
Database Link	Entrez Gene 11461 MouseEntrez Gene 81822 RatEntrez Gene 60 Human UniProt ID P60709
Background	The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al. (1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.
Synonyms	Actin cytoplasmic 1, Beta-Actin
Reference Data
Protein Families	ES Cell Differentiation/IPS
Protein Pathways	Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, Tight junction, Vibrio cholerae infection, Viral myocarditis

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