PCNA Mouse Monoclonal Antibody [Clone ID: IML-83]

Specifications

Product Data
Clone Name	IML-83
Applications	IF, IHC, WB
Recommend Dilution	Western Blot: 2 μg/ml. Immunohistochemistry on frozen and paraffin sections: 0.4 - 1 μg/ml. Immuocytochemistry.
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Protein A fusion protein
Specificity	This antibody reacts to PCNA.
Formulation	1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative. State: Purified State: Lyphilized purified Ig fraction
Reconstitution Method	Restore with 1.2% sodium acetate or neutral PBS
Concentration	0,1 mg/ml (after reconstitution with PBS)
Purification	Affinity chromatography
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at -20°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	proliferating cell nuclear antigen
Database Link	Entrez Gene 18538 MouseEntrez Gene 25737 RatEntrez Gene 5111 Human UniProt ID P12004
Background	Proliferating cell nuclear antigen (PCNA) was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferative state of the cell. PCNA /cyclin has been localized by in situ hybridization to the short arm of human chromosome 20 with a peak of grains over band 20p13. PCNA gene is present in single copy and has 6 exons. It spans 4,961 bp. Synthesis of the nuclear protein cyclin and DNA in quiescent mouse fibroblasts is coordinately induced by serum and purified growth factors. PCNA controls establishment of sister chromatid cohesion during S phase.
Synonyms	Cyclin
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency
Protein Pathways	Base excision repair, Cell cycle, DNA replication, Mismatch repair, Nucleotide excision repair

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