p53 (TP53) Mouse Monoclonal Antibody [Clone ID: IMD-53]

Specifications

Product Data
Clone Name	IMD-53
Applications	IF, IHC, WB
Recommend Dilution	Western Blot: 0.5-1 μg/ml.  Immunocytochemistry.  Immunohistochemistry on Frozen Sections: Use at 0.5-2 μg/ml to detect p53 in formalin or acetone fixed tissues.  Immunohistochemistry on Paraffin Sections: Use at 0.5-2 μg/ml to detect p53 in formalin fixed and paraffin embedded tissues.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human wild-type p53 protein.
Specificity	This antibody reacts to p53 (TP53).
Formulation	1.2% Sodium Acetate with 2 mg BSA and 0.01 mg Sodium Azide as preservative State: Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 1.2% Sodium Acetate or neutral PBS
Concentration	0.1 mg/ml (after reconstitution with PBS)
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at -20°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	tumor protein p53
Database Link	Entrez Gene 7157 Human UniProt ID P04637
Background	The p53 tumor antigen is found in increased amounts in a wide variety of transformed cells. The protein is also detectable in many actively proliferating, nontransformed cells, but it is undetectable or present at low levels in resting cells. This protein induces cell cycle arrest or apoptosis in response to sublethal or severe DNA damage, respectively, by differential transcription of target genes and through transcription-independent apoptotic functions. The p53 protein contains 393 amino acids. Human p53 tumour antigen is Locatedto band 17p13. p53 mutations are common in pancreatic cancer and are absent in chronic pancreatitis.
Synonyms	Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency, Transcription Factors
Protein Pathways	Amyotrophic lateral sclerosis (ALS), Apoptosis, Basal cell carcinoma, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, Glioma, Huntington's disease, MAPK signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer, Thyroid cancer, Wnt signaling pathway

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.