FLT4 Mouse Monoclonal Antibody [Clone ID: 2E11]

Specifications

Product Data
Clone Name	2E11
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA: 0.5-1.5 µg/ml, allows the detection of 0.25-0.5 ng/well rhVEGFR-3/FLT-4 in combination with compatible secondary reagents. Western Blot: 0.5-1 µg/ml with the appropriate secondary reagents to detect Human VEGFR-3/FLT-4. Depending on the visualisation method the detection limit for rh VEGFR-3/FLT-4 is approximately 5 ng/lane under reducing conditions. Immunofluorescence/Immunohistochemistry on Frozen Sections: 1-10 µg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human Vascular Endothelial Growth Factor Receptor 3 (rh VEGFR-3/FLT-4) extracellular domain.
Specificity	This antibody detects Human VEGFR-3/FLT-4. Other species not tested.
Formulation	PBS, pH 7.4 without preservatives State: Aff - Purified State: Lyophilized purified IgG fraction from Hybridoma Supernatant
Reconstitution Method	Restore in Sterile water to a concentration of 0.1-1.0 mg/ml
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	fms related tyrosine kinase 4
Database Link	Entrez Gene 2324 Human UniProt ID P35916
Background	VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk 1) and VEGFR-3 (FLT-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR-1 to -3 is almost exclusively restricted to hematopoietic precursor cells, vascular and lymphatic endothelial cells and to the monocyte/macrophage lineage. These receptors play essential roles in vasculogenesis, hematopoiesis, angiogenesis and lymphangiogenesis. The VEGFR-3 cDNA encodes a 1298 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGFR-3 is composed of a 751 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 482 aa residue cytoplasmic domain. Both VEGF-C and VEGF-D have been shown to bind and activate VEGF R3 (Flt-4). The Flt-4 gene is widely expressed in the early embryo but becomes restricted to the lymphatic endothelial a latter stage of development. It is important for lymphangiogenesis.
Synonyms	VEGFR3, FLT4, VEGF Receptor 3
Reference Data
Protein Families	Druggable Genome, Protein Kinase, Transmembrane
Protein Pathways	Cytokine-cytokine receptor interaction, Focal adhesion

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