EGFR (Extracell. non Ligand binding Site) Mouse Monoclonal Antibody [Clone ID: EGF-R2]

Specifications

Product Data
Clone Name	EGF-R2
Applications	ELISA, FC, IHC
Recommend Dilution	ELISA (Ref.1-3). Flow Cytometry. Immunocytochemistry (Ref.2). Immunohistochemistry on Cryosections. Immunohistochemistry on Paraffin Sections.  Antigen Retrieval: Heat the deparaffinized, rehydrated sections 2x in fresh citrate buffer pH 6.0 for 5 min. in microwave.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human EGF-Receptor
Specificity	Recognizes Human EGF-Receptor (extracellular non ligand binding site). There was no cross reactivity obtained with v-erb-B
Formulation	PBS, pH 7.4 containing 20 mM D-(+)-trehalose dihydrate, 40 mM D-(-)-mannitol State: Purified State: Lyophilized purifed IgG fraction from Cell Culture Supernatant
Reconstitution Method	Restore in aqua bidest to 1 mg/ml
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	epidermal growth factor receptor
Database Link	Entrez Gene 1956 Human UniProt ID P00533
Background	Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
Synonyms	Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
Reference Data
Protein Families	Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Secreted Protein, Stem cell relevant signaling - JAK/STAT signaling pathway, Transmembrane
Protein Pathways	Adherens junction, Bladder cancer, Calcium signaling pathway, Colorectal cancer, Cytokine-cytokine receptor interaction, Dorso-ventral axis formation, Endocytosis, Endometrial cancer, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, MAPK signaling pathway, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.