PAK4 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 6C1]

Specifications

Product Data
Clone Name	6C1
Applications	WB
Recommend Dilution	Immunoblotting: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated SW480 cells (See Protocols for more details).
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Peptide conjugated to hemocyanin Epitope: Peptide derived from the kinase activation loop.
Specificity	Recognizes p21 activated kinase 4 (PAK4).
Formulation	1ml PBS, containing 0.09% Sodium Azide/PEG and Sucrose. State: Purified State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subsequent Ultrafiltration and Size Exclusion Chromatography.
Conjugation	Unconjugated
Storage Condition	For long-term storage, freeze lyophilizate upon arrival (-20°C). Upon reconstitution, aliquote and freeze in liquid nitrogen; reconstituted antibody can be stored frozen at -80°C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months.
Gene Name	p21 (RAC1) activated kinase 4
Database Link	Entrez Gene 70584 MouseEntrez Gene 292756 RatEntrez Gene 10298 Human UniProt ID O96013
Background	p21 activated kinases (PAK) are regulated by the small GTP - binding proteins Rac and cdc42, which stimulate autophosphorylation and phosphorylation of downstream signaling proteins. PAK4 is activated upon autophosphorylation of Ser 474 in the kinase activation loop. PAK4 is frequently overexpressed in human tumor cell lines and might play an important role in oncogenesis.
Synonyms	PAK 4, PAK-4, p21-activated kinase 4, KIAA1142
Note	Mol. weight: 60 kDa Protocol: Positive Control Provided. Cell lysate from untreated SW480 cells Description: Cell lysate from untreated SW480 cells, colorectal adenocarcinoma (Human) Format: Lyophilized cell lysate from serum starved SW480 cells. Reconstitution:Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Axon guidance, ErbB signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway

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