LC3B (MAP1LC3B) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 2G6]
CAT#: AM20213PU-N
LC3B (MAP1LC3B) (N-term) (incl. pos. control) mouse monoclonal antibody, clone 2G6, Purified
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CNY 5,973.00
货期*
5周
规格
Cited in 1 publication. |
Specifications
Product Data | |
Clone Name | 2G6 |
Applications | IF, WB |
Recommend Dilution | Immunoblotting: 0.5 µg/ml for HRPO/ECL detection Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. We strongly recommend to use PVDF membranes for immunoblot analysis. Immunocytochemistry: Use at 1-10 µg/ml (Paraformaldehyd/Methanol fixation). Included Positive Control: Cell lysate from untreated Neuro 2A (See Protocol below). |
Reactivity | Hamster, Human, Monkey, Mouse, Rat |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | Synthetic peptide hemocyanin conjugated derived from the N-terminus of LC3-B |
Specificity | This antibody specifically recognizes both forms of endogenous LC3, the cytoplasmic LC3-I (18 kDa) as well as the lipidated form generated during autophagosome and autophagolysosome formation: LC3-II (16 kDa). |
Formulation | PBS containing 0.09% Sodium Azide, PEG and Sucrose/50% Glycerol State: Purified State: Liquid purified IgG fraction |
Concentration | lot specific |
Purification | Subsequent Ultrafiltration and Size Exclusion Chromatography |
Conjugation | Unconjugated |
Storage Condition | Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
Gene Name | microtubule associated protein 1 light chain 3 beta |
Database Link | |
Background | Autophagy is an alternative process of proteasomal degradation for some long-lived proteins or organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). Genetic studies in yeast have shown that Autophagy-defective Gene-8 (Atg-8) represents a specific marker for autophagy. Among the four families of mammalian Atg8-related proteins only LC3 (microtubule-associated protein1 light chain 3) is expressed at sufficient high levels and efficiently recruited to autophagic vesicles in cells and tissues. During autophagy the cytoplasmic form, LC3-I is processed and recruited to autophagosomes, where LC3-II is generated by site specific proteolysis near to the C-terminus. Autophagic vacuoles have been also reported frequently in cardiomyopathies or muscle cells exposed to different experimental settings. |
Synonyms | MAP1LC3B, MAP1A/MAP1B, Map1lc3b, Map1alc3, Map1lc3 |
Note | Molecular Weight: 18 kDa (LC3-I), 16 kDa (LC-II) Protocol: Positive Control: Cell lysate from untreated Neuro 2A cells, brain endothelioma (Mouse) Format: Lyophilized cell lysate from serum starved Neuro 2A. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation. Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing. |
Reference Data |
Citations (1)
The use of this Antibodies has been cited in the following citations: |
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BAG3 promotes autophagy and glutaminolysis via stabilizing glutaminase
,Zhao, S;Wang, JM;Yan, J;Zhang, DL;Liu, BQ;Jiang, JY;Li, C;Li, S;Meng, XN;Wang, HQ;,
Cell Death Dis
,PubMed ID 30910998
[MAP1LC3B]
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