Brk (PTK6) Mouse Monoclonal Antibody [Clone ID: 24B7]

CAT#: AM20211PU-N

Brk (PTK6) mouse monoclonal antibody, clone 24B7, Purified



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CNY 5,973.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Clone Name 24B7
Applications WB
Recommend Dilution Immunoblotting: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
Included Potitive Control: Cell lysate from untreated MDA-MB 468 cells (See Protocols for more details).
Reactivity Human
Host Mouse
Clonality Monoclonal
Immunogen Peptide conjugated to hemocyanin.
Epitope: Internal peptide derived from BRK/PTK6 sequence
Specificity Recognizes BRK/PTK6
Formulation PBS containing 0.09% Sodium Azide, PEG, Sucrose and 50% Glycerol.
State: Purified
State: Liquid purified IgG fraction.
Concentration lot specific
Purification Subsequent Ultrafiltration and Size Exclusion Chromatography.
Conjugation Unconjugated
Storage Condition Store the antibody in aliquots) at -20°C.
Avoid repeated freeze-thaw cycles.
Gene Name protein tyrosine kinase 6
Background This protein encoded is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. Its presence in the nucleus appears to be linked to suppression of tumor progression. The encoded protein has been shown to undergo autophosphorylation.
Synonyms Tyrosine-protein kinase 6, Breast tumor kinase
Note Molecular Weight: 52 kDa

Protocol: Positive Control: Cell lysate from untreated MDA-MB-468 cells, breast adenocarcinoma (Human)

Format: Lyophilized cell lysate from serum starved MDA-MB-468 cells.

Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.

Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells.
Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins.
Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation.

Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing.
Reference Data
Protein Families Druggable Genome, Protein Kinase, Secreted Protein
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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