Brk (PTK6) Mouse Monoclonal Antibody [Clone ID: 24B7]

Specifications

Product Data
Clone Name	24B7
Applications	WB
Recommend Dilution	Immunoblotting: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Included Potitive Control: Cell lysate from untreated MDA-MB 468 cells (See Protocols for more details).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Peptide conjugated to hemocyanin. Epitope: Internal peptide derived from BRK/PTK6 sequence
Specificity	Recognizes BRK/PTK6
Formulation	PBS containing 0.09% Sodium Azide, PEG, Sucrose and 50% Glycerol. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Subsequent Ultrafiltration and Size Exclusion Chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody in aliquots) at -20°C. Avoid repeated freeze-thaw cycles.
Gene Name	protein tyrosine kinase 6
Database Link	Entrez Gene 5753 Human UniProt ID Q13882
Background	This protein encoded is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. Its presence in the nucleus appears to be linked to suppression of tumor progression. The encoded protein has been shown to undergo autophosphorylation.
Synonyms	Tyrosine-protein kinase 6, Breast tumor kinase
Note	Molecular Weight: 52 kDa Protocol: Positive Control: Cell lysate from untreated MDA-MB-468 cells, breast adenocarcinoma (Human) Format: Lyophilized cell lysate from serum starved MDA-MB-468 cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation. Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing.
Reference Data
Protein Families	Druggable Genome, Protein Kinase, Secreted Protein

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