Beclin 1 (BECN1) Mouse Monoclonal Antibody [Clone ID: 12B4]

CAT#: AM20210PU-N

Beclin 1 (BECN1) mouse monoclonal antibody, clone 12B4, Purified



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CNY 5,973.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Clone Name 12B4
Applications WB
Recommend Dilution Immunoblotting: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
Included Potitive Control: Cell lysate from untreated Neuro 2A (See Protocols for more details).
Reactivity Canine, Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Immunogen Synthetic peptide conjugated to hemocyanin derived from Beclin sequence.
Specificity This Monoclonal antibody recognizes Beclin at 52 kDa in Western-blot application.
Formulation PBS containing 0.09% Sodium Azide, PEG, Sucrose and 50% Glycerol.
State: Purified
State: Liquid purified IgG fraction.
Concentration lot specific
Purification Subsequent Ultrafiltration and Size Exclusion Chromatography.
Conjugation Unconjugated
Storage Condition Upon receipt, store (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Predicted Protein Size 52 kDa
Gene Name beclin 1
Background Beclin 1 is a ubiquitous coiled-coil protein that plays a central role in autophagy. It interacts with several cofactors (Ambra1, Bif1 , UVRAG) to activate the lipid kinase Vps34, thereby inducing autophagy. In normal conditions, the BH3 domain of Beclin 1 is bound to, and inhibited by, Bcl2 or Bcl-X(L). This interaction can be disrupted by BH3-only proteins to induce autophagy. Nutrient starvation, a potent physiological inducer of autophagy, can stimulate the dissociation of Beclin 1 from its inhibitors, either by activating BH3-only proteins, such as Bad, or by post-translational modifications of Bcl2.
Beclin 1 may play a role in anti-viral host defence, and protects against infection by a neurovirulent strain of Sindbis virus. Beclin 1 is also a haploinsufficient tumour suppressor.
Genetic studies revealed that Beclin-1 has structural similarity to the yeast autophagy gene, atg6. Beclin-1 is part of a complex with class III PI3 kinase, Vsp34, that is responsible for autophagosome formation.
Synonyms BECN1, GT197
Note Protocol: Positive Control: Cell lysate from untreated Neuro2A cells, brain endothelioma (Mouse)

Format: Lyophilized cell lysate from serum starved Neuro 2A cells.

Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.

Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells.
Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins.
Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation.

Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing.
Reference Data
Protein Families Druggable Genome
Protein Pathways Regulation of autophagy
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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