Beclin 1 (BECN1) Mouse Monoclonal Antibody [Clone ID: 12B4]

Specifications

Product Data
Clone Name	12B4
Applications	WB
Recommend Dilution	Immunoblotting: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Included Potitive Control: Cell lysate from untreated Neuro 2A (See Protocols for more details).
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide conjugated to hemocyanin derived from Beclin sequence.
Specificity	This Monoclonal antibody recognizes Beclin at 52 kDa in Western-blot application.
Formulation	PBS containing 0.09% Sodium Azide, PEG, Sucrose and 50% Glycerol. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Subsequent Ultrafiltration and Size Exclusion Chromatography.
Conjugation	Unconjugated
Storage Condition	Upon receipt, store (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Predicted Protein Size	52 kDa
Gene Name	beclin 1
Database Link	Entrez Gene 56208 MouseEntrez Gene 114558 RatEntrez Gene 8678 Human UniProt ID Q14457
Background	Beclin 1 is a ubiquitous coiled-coil protein that plays a central role in autophagy. It interacts with several cofactors (Ambra1, Bif1 , UVRAG) to activate the lipid kinase Vps34, thereby inducing autophagy. In normal conditions, the BH3 domain of Beclin 1 is bound to, and inhibited by, Bcl2 or Bcl-X(L). This interaction can be disrupted by BH3-only proteins to induce autophagy. Nutrient starvation, a potent physiological inducer of autophagy, can stimulate the dissociation of Beclin 1 from its inhibitors, either by activating BH3-only proteins, such as Bad, or by post-translational modifications of Bcl2. Beclin 1 may play a role in anti-viral host defence, and protects against infection by a neurovirulent strain of Sindbis virus. Beclin 1 is also a haploinsufficient tumour suppressor. Genetic studies revealed that Beclin-1 has structural similarity to the yeast autophagy gene, atg6. Beclin-1 is part of a complex with class III PI3 kinase, Vsp34, that is responsible for autophagosome formation.
Synonyms	BECN1, GT197
Note	Protocol: Positive Control: Cell lysate from untreated Neuro2A cells, brain endothelioma (Mouse) Format: Lyophilized cell lysate from serum starved Neuro 2A cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation. Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing.
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Regulation of autophagy

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