Biotin Mouse Monoclonal Antibody [Clone ID: 2H2]

Specifications

Product Data
Clone Name	2H2
Applications	ELISA, IP, WB
Recommend Dilution	ELISA: Use at 0.05 µg/ml Immunoblotting: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Immunoprecipitation: Use at 1 µg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Biotin conjugated to hemocyanin.
Specificity	This antibody interacts with high affinity with biotinylated proteins.
Formulation	1 ml PBS / 0.09% Sodium Azide/PEG and Sucrose State: Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subsequent Ultrafiltration and Size Exclusion Chromatography
Conjugation	Unconjugated
Storage Condition	For long-term storage, freeze lyophilizate upon arrival (-20°C). Upon reconstitution, aliquote and freeze in liquid nitrogen. reconstituted antibody can be stored frozen at -80°C up to 1 year. Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months. Avoid repeated freeze-thaw cycles.
Background	Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
Synonyms	Vitamin B7, Vitamin H
Reference Data

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