CD229 (LY9) Mouse Monoclonal Antibody [Clone ID: HLy9.25]

Specifications

Product Data
Clone Name	HLy9.25
Applications	FC, FN, IF, IP, WB
Recommend Dilution	Flow cytometry: 6 µg/ml; Use peripheral blood lymphocytes as positive control. Immunoprecipitation. Western blot. Immunocytochemistry. Functional Application (regulation of activation).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	CD299-transfected 300.19 pre-B cell line
Specificity	The antibody recognizes CD229 / Ly9, a 100-120 kDa cell surface glycoprotein expressed on T and B cells.
Formulation	Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 State: Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein A affinity chromatography (> 95% pure by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	lymphocyte antigen 9
Database Link	Entrez Gene 4063 Human UniProt ID Q9HBG7
Background	CD229 (Ly9) is a cell surface receptor of the CD150 family, which includes also e.g. CD48 and CD224. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. High levels of CD229 are found on T and B cells, where its expression increases during their maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets and erythrocytes. CD229 has been also reported on mouse monocytes and NK cells. CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen presenting B cells during antigen-dependent immune synapse formation.
Synonyms	Ly-9
Reference Data
Protein Families	Transmembrane

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