CD11a / ITGAL Rat Monoclonal Antibody [Clone ID: M17/4]

Specifications

Product Data
Clone Name	M17/4
Applications	FC, FN, IHC, IP
Recommend Dilution	Flow Cytometry: 1 µg/ml. Immunoprecipitation. Immunohistochemistry on Frozen Sections (Acetone fixation). Positive Control: Mouse spleen or thymus. Functional Assays: Blocking.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	C57BL/6 mouse splenic secondary cytotoxic T lymphocytes
Specificity	The antibody reacts with CD11a (alpha-subunit of murine LFA-1), a 180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.
Formulation	PBS, pH~7.4 State: Aff - Purified State: Liquid purified Ig fraction (> 95% by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Purified by protein-G affinity chromatography.
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Database Link	UniProt ID P24063
Background	CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions.
Synonyms	Integrin alpha-L, LFA1, LFA-1
Reference Data

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