NANOG Mouse Monoclonal Antibody [Clone ID: 60CT70.5.12]

Specifications

Product Data
Clone Name	60CT70.5.12
Applications	IHC, WB
Recommend Dilution	ELISA: 1/1,000. Western blot: 1/1,000. Immunohistochemistry: 1/10-1/50.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	This antibody is generated from mouse immunized with Human recombinant NANOG protein.
Specificity	This antibody is specific to Human NANOG.
Formulation	PBS State: Purified State: Liquid purified Ig fraction Preservative: 0.09% (W/V) Sodium Azide
Concentration	lot specific
Purification	Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	34620 Da
Gene Name	Nanog homeobox
Database Link	Entrez Gene 79923 Human UniProt ID Q9H9S0
Background	NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cell proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.
Synonyms	FLJ12581; FLJ40451; hNanog
Reference Data

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