Influenza A (H0N1, H1N1, H2N2 and H3N2) Mouse Monoclonal Antibody [Clone ID: 9G8]

Specifications

Product Data
Clone Name	9G8
Applications	ELISA, IF, IHC, WB
Recommend Dilution	ELISA: 1/2000-1/10000. Western Blot: 1/200-1/1000.  Immunohistochemistry. Immunocytochemistry on infected cells (IF): 1/100-1/500.
Reactivity	Influenza A Virus
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant NP
Specificity	Reacts with NP of Influenza A virus group specific antigen (H0N1, H1N1, H2N2 and H3N2). No cross reaction with Influenza B or with other respiratory viruses.
Formulation	0.1M Tris, 0.1M Glycine, 2% Sucrose State: Purified State: Lyophilized powder Preservative: None
Reconstitution Method	Restore in distilled water.
Purification	Protein A Chromatography
Conjugation	Unconjugated
Storage Condition	Lyophilized power stable for a minimum of 2 years at -20°C. Store reconstituted antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Influenza type A viruses are divided into subtypes based on the antigenic differences of two viral surface proteins, hemagglutinin (H) and neuraminidase (N). On infection of the respiratory tract, the hemagglutinin molecule binds to sialic acid-containing receptors on the epithelial cells resulting in endocytosis. Once the virus has been engulfed, the hemagglutinin allows the viral membrane to fuse with the endosomal membrane. Neuraminidase functions to aid viral release from host cells by cleaving terminal sialic acid residues from carbohydrate moieties on the cell surface. Subtype antigenic variations result from a process known as antigenic drift whereby these surface proteins constantly mutate in order to evade the host immune repspone.
Synonyms	Influenza A Virus, Seasonal Flu
Reference Data

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