CD175 Mouse Monoclonal Antibody [Clone ID: TN218]

Specifications

Product Data
Clone Name	TN218
Applications	IF, IHC
Recommend Dilution	Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections and cell smears): 1/50-1/100 using streptavidin~biotin system or polymer system, incubate 30 minutes at room temperature. Immunofluorescence. Recommended Positive Control: Human gastrointestinal tumor.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Asialo ovine submaxillary mucin (AOSM).
Specificity	This antibody stains Tn antigen in epithelial carcinoma, pancreatic, colon, stomach, breast. Cellular Localization: Cytoplasmic and epithelial membrane.
Formulation	PBS, pH 7.4 containing 0.05% Sodium Azide as preservative. State: Supernatant State: Liquid concentrated cell culture medium.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Aberrant glycosylation is a hallmark of cancer cells. The blood group precursors T (Thomsen-Friedenreich) and Tn epitopes are shielded in healthy and benign-diseased tissues but uncovered in approx. 90% of carcinomas. T and Tn glycoproteins are specific, autoimmunogenic pancarcinoma antigens. These antigens may also be found in neoplastic blood cells Fundamental chemical and physical aspects of these glycoproteins of primary carcinomas are discussed first. Tn and T epitopes are cell and tissue adhesion molecules, essential in invasion by and metastasis of carcinoma which includes adherent and proliferative phases. Immunohistochemical studies of the extent of Tn antigen expression in primary breast carcinoma demonstrate it highly significant correlation with clinicopathological tumor stages, and hence its value as a reliable prognosticator.
Synonyms	Blood Group Tn Antigen
Reference Data

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