acidic Cytokeratin Mouse Monoclonal Antibody [Clone ID: AE1]

Specifications

Product Data
Clone Name	AE1
Applications	FC, IF
Recommend Dilution	Immunofluorescence: 10-20 µg/ml (1/10-1/20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4ºC. Flow Cytometry: 0.2-1.0 µg/0.1 ml (1/200-1/1,000) (Not tested in our lab).
Reactivity	Bovine, Chicken, Human, Monkey, Mouse, Porcine, Rabbit, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human epithelial keratin.
Specificity	This antibody recognizes low molecular weight cytokeratins (CK 10 (56.5), CK14 (50), CK15 (50), CK16 (48) and CK19(40) of the acidic family. Cellular Localization: Cytoplasmic.
Formulation	PBS, pH 7.4 Label: FITC State: Purified State: Liquid purified Ig fraction Stabilizer: 1% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A Chromatography
Conjugation	FITC
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Background	Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1, CK2, CK3, CK4, CK5, CK6, CK7, CK8 and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10, CK12, CK 13, CK14, CK16, CK17, CK18, CK19 and CK20.
Synonyms	Cytokeratin type I, Keratin type I, LMW Cytokeratins
Reference Data

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