Cytokeratin 19 (KRT19) Mouse Monoclonal Antibody [Clone ID: CK202]

Specifications

Product Data
Clone Name	CK202
Applications	IF, IHC
Recommend Dilution	Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections and cell smears). For IHC dilute concentared antibody at 1/50-1/100, use streptavidin~biotin system or polymer system, incubate 30 minutes at room temperature. FFPE tissue section requires antigen retrieval (boiling tissue in 10 mM citrate, pH 6.0 for 10-15 minutes followed by cooling for 10-15 minutes). Immunofluorescence: Recommended Positive Control: Breast ca, colon ca, and colon ca cell line, LS174T and HT29.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human mammary epithelium, solubilized by detergent.
Specificity	This antibody recognises Cytokeratin 19 (CK19). Cellular Localization: Cytoplasmic.
Formulation	PBS, pH 7.4 containing 0.05% Sodium Azide as preservative. State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	keratin 19
Database Link	Entrez Gene 3880 Human UniProt ID P08727
Background	Cytokeratin 19 is a type I acidic subfamily of intermediate filament protein that is expressed in stratified and simple type epithelia. Keratin 19 is not expressed in hepatocytes; therefore, antibody to keratin 19 is useful in the identification of liver metastasis. The degree of keratin 19 positivity in breast cancer distinguishes malignant from benign tumors. Keratin 19 is often co-expressed with keratin 7.
Synonyms	Cytokeratin-19, Keratin-19, KRT19, CK19, K19
Reference Data

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