HPRT (HPRT1) Mouse Monoclonal Antibody [Clone ID: AT2G8]

Specifications

Product Data
Clone Name	AT2G8
Applications	ELISA, WB
Recommend Dilution	ELISA. Western blot (1/500-1/5000). Recommended starting dilution is 1/1000.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human HPRT (1-218aa) purified from E. coli
Specificity	The antibody recognizes Human HPRT. Other species not tested.
Formulation	PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Protein-G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	hypoxanthine phosphoribosyltransferase 1
Database Link	Entrez Gene 3251 Human UniProt ID P00492
Background	HPRT is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. HPRT, which acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP, functions primarily to salvage purines from degraded DNA to renewed purine synthesis.
Synonyms	HGPRTase, HGPRT
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency
Protein Pathways	Drug metabolism - other enzymes, Metabolic pathways, Purine metabolism

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