GAPDH (Loading Control) Mouse Monoclonal Antibody [Clone ID: AT8G4]

Specifications

Product Data
Clone Name	AT8G4
Applications	ELISA, IF, WB
Recommend Dilution	ELISA: 1/1000. Western blot: 1/500-1/10,000. Immunofluorescence: 1/500-1/10,000.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human GAPDH (1-335aa) purified from E. coli.
Specificity	The antibody recognizes Human GAPDH. Other species not tested.
Formulation	PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Protein-G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	glyceraldehyde-3-phosphate dehydrogenase
Database Link	Entrez Gene 2597 Human UniProt ID P04406
Background	Multiple roles for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) have been recently appreciated. GAPDH is found in the particulate fractions, such as the nucleus, the mitochondria, and the small vesicular fractions. GAPDH gene expression is specifically increased during programmed neuronal cell death. When cells are exposed to various stressors, dynamic subcellular re-distribution of GAPDH occurs. GAPDH is also involved in various diseases, especially neurodegenerative disorders and cancers. As a membrane protein, GAPDH functions in endocytosis; in the cytoplasm, it is involved in the translational control of gene expression; in the nucleus, it functions in nuclear tRNA export, in DNA replication, and in DNA repair.
Synonyms	GAPD, CDABP0047
Reference Data
Protein Families	ES Cell Differentiation/IPS
Protein Pathways	Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways

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