CREB1 pSer133 Mouse Monoclonal Antibody [Clone ID: B1C4]

Specifications

Product Data
Clone Name	B1C4
Applications	ELISA
Recommend Dilution	Indirect ELISA: The antibody is reactive to Serine133 phosphorylated Human CREB peptide and reactive to Serine133 phosphorylated Human CREB peptide conjugated with KLH or BSA. The suggested antigen coating quantity is 1 µg/ml of peptide/or peptide conjugating in pH 9.5 Carbonate buffer, 100 µl/well.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Phosphorylated peptide fragment of CREB (pSer133), conjugated with KLH
Specificity	Reactive to serine133 phosphorylated Human CREB peptide fragment, and reactive to KLH or BSA conjugated Serine133 phosphorylated Human CREB peptide fragment. Not reactive to un-conjugated KLH or BSA.
Formulation	0.01 M PBS, pH 7.0 without preservatives. State: Aff - Purified State: Lyophilized purified IgG fraction.
Reconstitution Method	Restore with Double distillated water to adjust the final concentration to 1.0 mg/ml.
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Upon receipt, store (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	cAMP responsive element binding protein 1
Database Link	Entrez Gene 1385 Human UniProt ID P16220
Background	Transcription factor CREB binds the cAMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth or neurotrophic factors. Protein kinase A and the calmodulin-dependent protein kinase CaMKII stimulate CREB phosphorylation at Ser133, a key regulatory site controlling transcriptional activity. Phosphorylation at Ser133 is also mediated by p44/42 MAP kinase, p90RSK, p38 MAP kinase and MSK1. CREB appears to play an important role in learning and memory in both flies and mice.
Synonyms	CREB-1
Reference Data
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Antigen processing and presentation, Huntington's disease, Melanogenesis, Prostate cancer

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