alpha 1 Fetoprotein (AFP) Mouse Monoclonal Antibody [Clone ID: AFP-Y1]

Specifications

Product Data
Clone Name	AFP-Y1
Applications	ELISA, IP, R, WB
Recommend Dilution	Western blot: The antibody identifies 68kD AFP. Radioimmunoassay. ELISA: This monoclonal AFP antibody can be used as a Capture antibody in a Sandwich ELISA for Human AFP Detection in combination with a HRP labeled tracer antibody. Suggested Capture Coating Dose: 0.1 µg/well. Substrate: TMB Purification of natural Human AFP. Immunoprecipitation (Qualitative and quantitative).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human AFP, which was isolated from amniotic fluid and purified by Affinity Chromatography.
Specificity	This antibody reacts with Human AFP. No detectable cross-reaction with Human normal serum proteins.
Formulation	0.01M PBS, pH 7.0 without preservatives. State: Aff - Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with Double distillated water to adjust the final concentration to 1.0 mg/ml.
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. After reconstitution, aliquot and store at -20°C.
Gene Name	alpha fetoprotein
Database Link	Entrez Gene 174 Human UniProt ID P02771
Background	Alpha Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries.
Synonyms	Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP
Reference Data
Protein Families	Druggable Genome, Secreted Protein

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