Helicobacter pylori (CagA Protein) Mouse Monoclonal Antibody [Clone ID: 3C10]

Specifications

Product Data
Clone Name	3C10
Applications	ELISA
Recommend Dilution	ELISA: Helicobacter pylori antibody (Clone 3C10) can be used as Detection antibody. In Sandwich ELISA, anti-HP CagA antibody clone 10E9 (Cat.-No AM09221PU-N) and anti-HP CagA antibody clone 5H10 (Cat.-No AM09222PU-N) coated wells match with HRP conjugated clone 3C10 to detect the recombinant antigen. HRP conjugated 3C10 also selectively detected cell lysate of a CagA containing HP strain when clone 10E9 was used as coating antibody.
Reactivity	Helicobacter pylori
Host	Mouse
Clonality	Monoclonal
Immunogen	A highly immunogenic 32Kd CagA fragment, recombinant.
Specificity	This antibody is raised against the H. pylori cytotoxin associated gene A protein (CagA). Reacts with the 32Kd recombinant Helicobacter pylori CagA. Cross reaction with other proteins has not been identified.
Formulation	0.01M PBS, pH 7.0 without preservatives State: Aff - Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with Double distillated water to adjust the final concentration to 1.0 mg/ml.
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Upon receipt, store (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Background	Helicobacter pylori (H. pylori), a spiral rod shaped gram-negative bacterium, is frequently found in the stomach. In infected populations, 10-20% may develop gastritis and gastric ulcer and 1-2% may develop cancer. The genome of H. pylori isolates from carriers with symptoms contains a 40kb pathogenicity island encoding H. pylori cytotoxin, cytotoxin associated gene A protein (CagA) and other virulence associated factors. CagA is used as a biomarker for virulent H. pylori strains.
Synonyms	H. pylori
Reference Data

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