Helicobacter pylori (CagA Protein) Mouse Monoclonal Antibody [Clone ID: 10E9]

Specifications

Product Data
Clone Name	10E9
Applications	ELISA, WB
Recommend Dilution	ELISA: HP CagA antibody (Clone 10E9) can be used as Capture antibody in Sandwich ELISA. In Sandwich ELISA, anti-HP CagA antibody clone 5H10 (Cat.-No AM09222PU-N) coated wells matches with HRP conjugated clone 5C6, clone 3C10 and clone 3C1 to detect the recombinant antigen. In addition, this clone selectively detected cell lysate of a CagA containing HP stain when HRP conjugated clone 3C10 was used. Western Blotting: This clone detected a 32 KD band corresponding to the molecular weight of the recombinant CagA antigen (immunogen).
Reactivity	Helicobacter pylori
Host	Mouse
Clonality	Monoclonal
Immunogen	A highly immunogenic 32 kD CagA fragment, recombinant.
Specificity	This antibody is raised against the H. pylori cytotoxin associated gene A protein (CagA). Reacts with the 32Kd recombinant Helicobacter pylori CagA. Cross reaction with other proteins has not been found.
Formulation	0.01M PBS, pH 7.0 without preservatives State: Aff - Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with Double distillated water to adjust the final concentration to 1.0 mg/ml.
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Upon receipt, store (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Background	Helicobacter pylori (H. pylori), a spiral rod shaped gram-negative bacterium, is frequently found in the stomach. In infected populations, 10-20% may develop gastritis and gastric ulcer and 1-2% may develop cancer. The genome of H. pylori isolates from carriers with symptoms contains a 40kb pathogenicity island encoding H. pylori cytotoxin, cytotoxin associated gene A protein (CagA) and other virulence associated factors. CagA is used as a biomarker for virulent H. pylori strains.
Synonyms	H. pylori
Reference Data

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