HCV NS3 Mouse Monoclonal Antibody [Clone ID: 20-8]

Specifications

Product Data
Clone Name	20-8
Applications	ELISA, WB
Recommend Dilution	ELISA: React with human Hepatitis C Virus. Western Blot: Use of Hepatitis C Virus NS3 antibody (clone 20-8) at 1 µg/ml will allow visualization of 0.1 ug/lane of synthetic NS-3 protein under both reducing and non-reducing condition.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	A highly antigenic polypeptide consisting of essential sequences of at least 60 residues in length, which were selected from genes encoding the NS-3 region of Chinese HCV strains.
Specificity	Recognizes Synthetic HCV NS-3 Protein (aa 1378-1458). No cross reaction with HCV capsid region and other non-structural regions.
Formulation	0.01M PBS, pH 7.0 without preservatives. State: Aff - Purified State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with Double distillated water to adjust the final concentration to 1.0 mg/ml
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Background	HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.
Synonyms	Hepatitis C Virus Serine proteiase NS3
Reference Data

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