TNF alpha (TNF) Mouse Monoclonal Antibody [Clone ID: B1E4]

Specifications

Product Data
Clone Name	B1E4
Applications	ELISA, WB
Recommend Dilution	ELISA: The detection limit of ELISA can reach 2.4 pg/ml when clone B1E4 is used as Tracer and clone CH8820 Cat.-No DM1049 is used as Coating antibody. Western Blotting: The antibody reacts with recombinant 17kDa TNF alpha.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant Human TNF alpha.
Specificity	This antibody recognizes both, recombinant and native TNF-alpha.
Formulation	0.01M PBS, pH 7.0 without preservatives. State: Aff - Purified State: Lyophilized purified IgG fraction.
Reconstitution Method	Restore with double distillated water to adjust the final concentration to 1.0 mg/ml.
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	tumor necrosis factor
Database Link	Entrez Gene 7124 Human UniProt ID P01375
Background	Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
Synonyms	TNF, TNF-a, TNFA, TNFSF2, Cachectin
Reference Data
Protein Families	Druggable Genome, Secreted Protein, Transcription Factors, Transmembrane
Protein Pathways	Adipocytokine signaling pathway, Allograft rejection, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Asthma, Cytokine-cytokine receptor interaction, Dilated cardiomyopathy, Fc epsilon RI signaling pathway, Graft-versus-host disease, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Natural killer cell mediated cytotoxicity, NOD-like receptor signaling pathway, RIG-I-like receptor signaling pathway, Systemic lupus erythematosus, T cell receptor signaling pathway, TGF-beta signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus, Type II diabetes mellitus

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