IL8 (CXCL8) Mouse Monoclonal Antibody [Clone ID: I8-61]

Specifications

Product Data
Clone Name	I8-61
Applications	ELISA, FN, WB
Recommend Dilution	ELISA: React with Human IL-8. Neutralizing: In chemotaxis assay, the antibody inhibited 97% of the chemotactic activity of IL-8 on RB/293 cell using 5 Ug/ml of MAb and 10ng/ml of Human IL-8 in assay. This MAb inhibited the chemotactic effect of Human IL-8, had no inhibitory effect to MIP 1-bata and RANTES in neutrophil chemotaxis assay. It indicates the MAb is special neutralizing the IL-8 activity. The MAb has been shown the neutralizing activity on calcium ion changes in Human granulocytes using FACS. Western Blot: Concentration of 0.02-0.1 µg/ml of this antibody will allow visualization of 100 ng/lane of Human IL-8.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant human IL-8.
Specificity	This monoclonal IL8 antibody recognizes both recombinant and native Human Interleukin-8. It does not cross react with Human Monocyte Chemotactic Activating Factor (MCAF) or RANTES (Regulated on Activation, Normal T-cell Expressed, and Secreted).
Formulation	0.01M PBS, pH 7.0 without preservatives State: Azide Free State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with Double distillated water to adjust the final concentration to 1.0 mg/ml.
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	C-X-C motif chemokine ligand 8
Database Link	Entrez Gene 3576 Human UniProt ID P10145
Background	Interleukin 8 (IL-8), formerly called monocyte-derived neutrophil chemotactic factor, belongs to the C-X-C chemokine family. IL-8 has 4 cysteine residues, as do other members of the chemokine family, and the first two cysteine residues are separated by glutamine. IL-8 consists of 72 amino acids with a molecular weight of 8,000 daltons. IL-8 exhibits chemotactic activity in vitro for T cells, basophils and neutrophils. IL-8 activates neutrophils to release lysosomal enzymes including myeloperoxidase, -mannosidase and -glucuronidase. IL-1 induces the production of IL-8 from fibroblasts, keratinocytes, endothelial cells, hepatoma cells, astrocytoma cells, glioblastoma cells, lung epithelial cells, synovial membrane cells, melanocytes, melanoma cells and gastric cancer cells. Lipopolysaccharides will stimulate IL-8 production in monocytes/macrophages, and expressed on endothelial cells in response to inflammation.
Synonyms	CXCL8, Protein 3-10C, Emoctakin, GCP1, MDNCF, MONAP, NAP1
Reference Data
Protein Families	Druggable Genome, Secreted Protein, Transmembrane
Protein Pathways	Bladder cancer, Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Epithelial cell signaling in Helicobacter pylori infection, NOD-like receptor signaling pathway, Pathways in cancer, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway

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