EGF Mouse Monoclonal Antibody [Clone ID: S-146]

Specifications

Product Data
Clone Name	S-146
Applications	ELISA
Recommend Dilution	ELISA: This HRP-conjugated Monoclonal EGF antibody can be used as a tracer/detection antibody in a Sandwich ELISA for Human EGF detection in combination with a Capture antibody Clone S-134 (Cat.-No DM1012). Suggested Capture Coating Dose: 0.3 µg/well. Substrate: TMB. If the above suggested conditions are followed approximately 20 pg/mL of EGF in serum/plasma.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant Human EGF, 6kDa, 53 amino acid residues.
Specificity	Reactive with natural and recombinant Human EGF. Does not cross react with other Human cytokines tested such as IL-1beta, IL-8, MCAF, TGF-beta and SAA.
Formulation	0.01M PBS, pH 7.0 ± 0.1 in 50% Glycerol and 0.01% Thimerosal as a bacteriostat Label: HRP State: Liquid purified IgG fraction Label: Horseradish Peroxidase
Purification	Affinity Chromatography on Protein G
Conjugation	HRP
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	epidermal growth factor
Database Link	Entrez Gene 1950 Human UniProt ID P01133
Background	Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein. Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development.
Synonyms	Urogastrone, Epidermal growth factor, URG, HOMG4
Reference Data
Protein Families	Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transmembrane
Protein Pathways	Bladder cancer, Cytokine-cytokine receptor interaction, Endocytosis, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton

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