Cofilin 1 (CFL1) Mouse Monoclonal Antibody [Clone ID: AT1C1]

Specifications

Product Data
Clone Name	AT1C1
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA. Western blot (1/1,000-1/2,000). Immunohistochemistry on Paraffin Sections (10 µg/ml). This Cofilin 1 antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human Cofilin-1 (aa 1-166) purified from E. coli
Specificity	The antibody recognizes Human Cofilin-1. Other species not tested.
Formulation	PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Protein-G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	cofilin 1
Database Link	Entrez Gene 1072 Human UniProt ID P23528
Background	Cofilin is a family of actin-binding proteins which disassembles actin filaments. It is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.
Synonyms	CFL1, CFL, p18
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton

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