Nuclei (Nuclear Marker) Mouse Monoclonal Antibody [Clone ID: 235-1]

Specifications

Product Data
Clone Name	235-1
Applications	FC, IF, IHC, IP
Recommend Dilution	Flow Cytometry: 1-2ug/million cells Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Immunocytochemistry (Acetone-fixed cells): 1-2ug/ml for 30 minutes at RT Immunohistochemistry (Frozen): 1-2ug/ml for 30 minutes at RT Optimal dilution for any specific application should be determined. Recommended Positive Control: Tonsil
Reactivity	Human, Primate
Host	Mouse
Clonality	Monoclonal
Immunogen	Nuclei of human myeloid leukemia biopsy cells.
Specificity	This Monoclonal Antibody is part of a new panel of rea gents, which recognizes subcellular organelles or compartments of Human cells. These marker s may be useful in identification of these organelles in cells, tissues, and biochemica l preparations. Monoclonal Antibody 235-1 recognizes an antigen associated with the nuclei in Human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear ma rker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. Cellular Localization: Nuclei. Negative Species: Mouse, Rat, Chicken.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Predicted Protein Size	70 kda and 80 kDa
Background	Hepatoblastoma is the most common primary tumor of the liver in children. The use of specific hepatocyte markers and also of alpha Fetoprotein or carcinoembryonic antigen are useful for the identification of normal and malignant fetal hepatocytes.
Synonyms	Human Nuclear Antigen
Reference Data

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