Il3 Rat Monoclonal Antibody [Clone ID: MP2-8F8]

Specifications

Product Data
Clone Name	MP2-8F8
Applications	ELISA, FC, IF
Recommend Dilution	ELISA: Useful useful as a Capture antibody in a Sandwich ELISA for quantifying Mouse IL-3 protein levels. (Ref.1-5) This antibody should be paired with purified recombinant mouse IL-3 as the standard. Immunofluorescence/Flow Cytometry: This antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-3-positive cells within mixed cell populations. Recombinant mouse IL-3 (Cat#AM08179PU-N) should be used as a Specificity Control.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	COS-expressed recombinant mouse IL-3.
Specificity	This antibody is specific to Mouse Interleukin-3/IL-3. (Ref.1-3)
Formulation	100 mM Borate buffered saline, pH 8.2 without preservatives or amine-containing buffer salts. State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	interleukin 3
Database Link	Entrez Gene 16187 Mouse UniProt ID P01586
Background	The pleiotropic IL3 (Interleukin 3) is a 15kDa cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells. IL3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL3 has also been shown to protect mast cells from undergoing apoptosis.
Synonyms	IL-3, Multipotential colony-stimulating factor, Hematopoietic growth factor, P-cell-stimulating factor, MCGF
Reference Data

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