LOC105369261 Mouse Monoclonal Antibody [Clone ID: DF-T1]

Specifications

Product Data
Clone Name	DF-T1
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	ELISA (Use Free BSA Antibody for coating).  Flow Cytometry: 0.5-1 µg/106 cells.  Immunofluorescence: 1-2 µ/ml.  Western Blot: 0.5-1 µg/ml.  Immunoprecipitation: 1-2 µg/500 µg protein lysate.  Immunohistochemistry on Frozen and Formallin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.  Positive Control: Paracortex in a tonsil or a reactive lymph node.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Myeloblastic KG1 cells.
Specificity	This Monoclonal antibody recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43 [Workshop IV]. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma. Cellular Localization: Cell Surface.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Predicted Protein Size	95, 115, or 135 kDa
Database Link	Entrez Gene 105369261 Human UniProt ID P16150
Background	CD43 is a cell surface glycoprotein which is expressed on all thymocytes and T-cells. CD43 is involved in activation of T cells, B cells, NK cells, and monocytes. CD43, which is also known as leukosialin, galactoglycoprotein and sialophorin. CD43 is a sialomucin which like many mucins can have both adhesive and anti-adhesive functions. Expression of CD43 is found on most leukocytes except resting B lymphocytes. Proteolytic processing upon activation decreases surface expression. CD43 is involved in activation of T cells, B cells, NK cells, and monocytes. The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages.
Synonyms	Leukocyte sialoglycoprotein, Sialophorin, Galactoglycoprotein, SPN
Reference Data

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