Bu-1b Mouse Monoclonal Antibody [Clone ID: 5-11G2]

Specifications

Product Data
Clone Name	5-11G2
Applications	FC, IHC, IP
Recommend Dilution	Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1) Immunoprecipitation. (Ref.1) Immunohistochemistry on Frozen Sections. (Ref.3)
Reactivity	Chicken
Host	Mouse
Clonality	Monoclonal
Immunogen	Bursal cells from 2- to 5-week-old CHA (Bu-1b) strain Chickens.
Specificity	This antibody recognizes Chicken Bu-1b alloantigen (Mr. 70 kDa). It reacts with lymphoid cells from CHA and H.B14Ab strains but does not react with cells from H.B14Aa.
Formulation	100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added. State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID Q90747
Background	Bu1b is expressed on chicken B cells throughout most of their development, as well as on some non lymphoid cells. It has no recognizable similarity to known mammalian molecules and therefore represents a unique B cell marker. Its presence in chickens may be related to differences in the properties of B cell development between chickens and mammalian species. Chicken Bu1b, a product of the Bu1b allele, is expressed on the cell surface as a disulfide linked homodimer. In 10 week old H.B15 and H.B2 chickens, Bu1 is found on 85 to 90% of bursal cells, 2 to 8% of thymocytes, 15 to 27% of spleen cells, and 2 to 18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In chickens heterozygous for the Bu1 alleles (Bu1 a/b), Bu1a does not exhibit allelic exclusion.
Synonyms	B6.2, chicken B-cell marker
Reference Data

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