LOC396098 Mouse Monoclonal Antibody [Clone ID: 21-1A4]

Specifications

Product Data
Clone Name	21-1A4
Applications	FC, IHC, IP
Recommend Dilution	Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1) Immunoprecipitation. (Ref.1) Immunohistochemistry on Frozen Sections. (Ref.3)
Reactivity	Chicken
Host	Mouse
Clonality	Monoclonal
Immunogen	Bursal cells from one-day-old H.B15 (Bu-1a/b) strain chickens.
Specificity	This antibody recognizes Chicken Bu-1a alloantigen (Mr. 70 kDa). It does not react with cells from CHA and H.B15 strains by Immunofluorescence.
Formulation	100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added. State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	B6.1
Database Link	Entrez Gene 396098 Chicken UniProt ID Q90746
Background	Chicken Bu-1a, a product of the Bu-1a allele, is expressed on the cell surface as a disulfide-linked homodimer. In 10-week-old H.B15 and H.B2 chickens, Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In Chickens heterozygous for the Bu-1 alleles (Bu-1a/b), Bu-1a does not exhibit allelic exclusion.
Synonyms	B-Cell Marker
Reference Data

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