CD28 Mouse Monoclonal Antibody [Clone ID: AV7]

Specifications

Product Data
Clone Name	AV7
Applications	FC, FN, IP
Recommend Dilution	Flow Cytometry. (Ref.1) Immunoprecipitation. (Ref.1) In vitro T-cell costimulation. (Ref.2)
Reactivity	Chicken
Host	Mouse
Clonality	Monoclonal
Specificity	This antibody recognizes Chicken CD28, a monomeric molecule of Mr 40-44 kDa with 50% amino acid sequence identity with mammalian CD28.
Formulation	100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added. State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD28 molecule
Database Link	Entrez Gene 396249 Chicken UniProt ID P31043
Background	Chicken CD28 antigen is a type I transmembrane monomeric glycoprotein which does not contain the cysteine residues that form disulfide-linked covalent homodimers found in the mammalian counterpart. This may reduce the avidity of binding to individual ligand molecules expected to be present on Ag presenting cells. Peripheral gamma/delta+ T cells are CD28-negative. (Ref.1) Monoclonal antibodies against CD28 have a costimulatory effect on T cells stimulated by phorbol myristate acetate (PMA), concanavalin A or MAbs against the avian T cell receptor (TCR). Both Vbeta1- and Vbeta2-expressing cells respond equally well to stimulation with anti-CD28 in combination with PMA. These responses are resistant to cyclosporin A, but inhibited by herbimycin A, suggesting that CD28 employs a signalling pathway at least partly distinct from that triggered by TCR/CD3. (Ref.2)
Synonyms	TP44
Reference Data

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