CD8A Mouse Monoclonal Antibody [Clone ID: 76-2-11]

Specifications

Product Data
Clone Name	76-2-11
Applications	CT, FC, IHC, IP
Recommend Dilution	Flow Cytometry – Quality tested 1,11-15,17-28 Immunohistochemistry-Frozen Sections – Reported in literature 2-6,16 Immunohistochemistry-Paraffin Sections – Reported in literature 7 Immunoprecipitation – Reported in literature 1 Blocking – Reported in literature 8,9 Depletion – Reported in literature 10 Complement Mediated Cell Depletion – Reported in literature 1
Reactivity	Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Fresh dd miniature swine thymocytes
Specificity	This antibody reacts with Porcine CD8 alpha (Mr. 32-34 kDa). (Ref-1-3)
Formulation	100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added. State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	Entrez Gene 396627 Pig UniProt ID Q6R2U9
Background	The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. CD8 is expressed on most thymocytes and approximately 1/3 of peripheral blood T cells in humans. CD8 alpha/beta heterodimers are expressed only on TCR alpha/beta T cells, whereas CD8 alpha homodimers can be expressed on alpha/beta and gamma/delta T cells and some NK cells in humans. The pattern of expression in other species has not been well documented.
Synonyms	CD8 alpha chain, CD8A, MAL
Reference Data

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