Lamp1 Rat Monoclonal Antibody [Clone ID: 1D4B]

Specifications

Product Data
Clone Name	1D4B
Applications	EM, FC, IHC, IP, WB
Recommend Dilution	Flow cytometry: < / = 1 µg/10e6 cells. (Ref.5) Immunohistochemistry on Frozen Sections. (Ref.5) Immunoaffinity Chromatography. (Ref.2) Western blot. (Ref.5) Immunoprecipitation. (Ref.1,3) Immunoelectron Microscopy. (Ref.6)
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Plasma membrane fraction of mouse embryo NIH3T3 cell line. (Ref.1)
Specificity	This antibody is specific to CD107a/LAMP-1 (Mr 110-140 kDa with a core protein of Mr ~40 kDa).
Formulation	100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added. State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	lysosomal-associated membrane protein 1
Database Link	Entrez Gene 16783 Mouse UniProt ID P11438
Background	CD107a, also known as lysosomal-associated membrane protein 1 (LAMP-1), is a heavily glycosylated, type I transmembrane protein that constitutes one of the two major sialoglycoproteins on lysosomal membranes that can be used to distinguish lysosomes from other organelles. (Ref.1) It is a ligand for galaptin, an S-type lectin present in extracellular matrix, through its recognition of acetyllactosamine oligosaccharide chains, and is a ligand for E-selectin-mediated cell adhesion. (Ref.2) CD107a is principally expressed in epithelial cells and macrophages in a variety of organs in normal and Beige mutant mice. (Ref.3) LAMP-1 may function in protecting the inner surface of the lysosomal membrane by forming a barrier to lysosomal hydrolases. (Ref.4)
Synonyms	LAMP-1, LAMPA, LGP120
Reference Data

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