Cd86 Rat Monoclonal Antibody [Clone ID: 2D10]

Specifications

Product Data
Clone Name	2D10
Applications	FC, FN, IHC, IP
Recommend Dilution	ELISA. (Ref.4) Flow Cytometry: Identification and enumeration of CD86+ cells. (Ref.1,2,4,6,7) Immunoprecipitation (Ref.1) Immunohistochemistry on Frozen Sections: Identification of CD86+ cells. (Ref.4). In vivo and in vitro blocking assays. (Ref.1-3,5)
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Mouse B cell lymphoma cell line 5C2.
Specificity	This antibody recognises CD86, the B7-2 costimulatory molecule and a ligand for CD28 and CD152.
Formulation	100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added. State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD86 antigen
Database Link	Entrez Gene 12524 Mouse UniProt ID P42082
Background	CD86, also known as B72, is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell costimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease. CD86 interacts with HHV8 (KSHV) MIR2 protein.
Synonyms	CD28LG2, Activation B7-2 antigen, B70, BU63, B7.2, FUN-1
Reference Data

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