CD103 Hamster Monoclonal Antibody [Clone ID: 2E7]

Specifications

Product Data
Clone Name	2E7
Applications	FC, FN, IHC, IP
Recommend Dilution	Flow Cytometry: Identification and enumeration of CD103 + cells. Immunoprecipitation: This antibody exhibits antigen immunoprecipitation patterns similar if not identical to the Rat anti-Mouse CD103 mAb M290. (Ref.1) Immunohistochemistry on Frozen Sections. In vitro Activation. See Ref.1 for more details.
Reactivity	Mouse
Host	Hamster
Clonality	Monoclonal
Specificity	This antibody is specific to CD103/alpha IEL beta7 integrin complex (Mr. 160 and 115 kDa peptides). This antibody is reported to have signal inducing activity in a redirected lysis assay and to co stimulate IEL and CD8+ lymph node cells in conjunction with anti TCR in vitro, which runs counter to the usual inhibition of CTL by anti integrins. The property of costimulation is not unique to the mouse since the combination of anti human alpha IEL (intraepithelial lymphocytes) plus anti TCR will produce similar proliferation in human cells, with the exception that CD8+ cells are not stimulated. (Ref.5)
Formulation	100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added. State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	integrin alpha E, epithelial-associated
Database Link	Entrez Gene 16407 Mouse UniProt ID Q60677
Background	CD103/Integrin alpha E is a new member of the integrin series of adhesion molecules containing the beta7 subunit. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and ~20% of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs, appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis and periphery. (Ref.1-3) In non-epithelial CD103+ T cells there is a bias toward expression on CD8+ cells. Integrin alpha E is a heterodimer comprising a 150kDa alpha chain and a 120kDa beta chain. Integrin alpha E is expressed by approximately 1% of peripheral blood lymphocytes, activated T lymphocytes and by hairy cell leukaemia cells. The antigen is also expressed by intraepithelial lymphocytes.
Synonyms	Integrin alpha-E, Integrin alpha-IEL, HML-1 antigen, ITGAE
Reference Data

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