Hspa5 Rat Monoclonal Antibody [Clone ID: 76-E6]

Specifications

Product Data
Clone Name	76-E6
Applications	ELISA, FN, WB
Recommend Dilution	ELISA. (Ref.6) Western blotting. (Ref.6,8) Studies of Immunoglobulin assembly and secretion. Ref.1,2)
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Specificity	This antibody is specific to BIP (Immunoglobulin heavy chain binding protein, Mr 78 kDa). Monoclonal antibody 76-E6 recognizes a conserved epitope localized within the region of amino acids 497 to 581 of BiP. (Ref.8)
Formulation	100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added. State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	heat shock protein 5
Database Link	Entrez Gene 14828 Mouse UniProt ID P20029
Background	The immunoglobulin heavy chain binding protein BiP (Binding Protein) is a member of the hsp70 family of heat shock proteins, and is identical to the glucose regulated protein grp78. (Ref.2) While BiP was originally described for its function in B cells, it is now known to be distributed in a variety of tissues, if not ubiquitous. The highly conserved hsp 70 proteins have an essential physiological role in stress responses and as Molecular Chaperones which are responsible for a variety of functions such as protein transport, prevention of protein toxicity and direction of protein folding. (Ref.1-5) With regard to its immunological role, BiP is a component of the endoplasmic reticulum and binds free intracellular heavy chains in nonsecreting pre-B cell lines (µ+,L-) or incompletely assembled Ig precursors in H+L+ secreting hybridomas and myelomas. In the absence of light chain synthesis, heavy chains remain associated with BiP and are not secreted. BiP is an ATP binding protein and the dissociation of the BiP-heavy chain complex is probably driven by the ATPase activity attributed to BiP. (Ref.7)
Synonyms	78 kDa glucose-regulated protein, Heat shock 70 kDa protein 5, BiP
Reference Data

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