Cd3e Rat Monoclonal Antibody [Clone ID: C363.29B (YCD3-1)]

Specifications

Product Data
Clone Name	C363.29B (YCD3-1)
Applications	FC, FN, IHC, IP
Recommend Dilution	Flow Cytometry (Ref.1,7-12). Immunoprecipitation (Ref.1) Functional Assay: In vitro Depletion of CD3+ cells. (Ref.1) In vitro Activation of T cells (accessory cells must be provided). (Ref.1,4) Immunohistochemistry on Froszen Sections (Ref.2-4). Immunohistochemistry on Paraffin Sections (Ref.5,6).
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	IL-4 producing Th2 cell lines including D10
Specificity	This antibody is specific to CD3e (Mr. 25 kD). The monoclonal antibody C363.29B recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex. (Ref.1) In the presence of Fc receptor-bearing accessory cells, soluble C363.29B can activate primed and naïve T cell in vitro. Immobilized C363.29 monoclonal antibody can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. (Ref.1,3,4) The monoclonal antibody is cytolytic, easily used for cell surface staining and a good immunoprecipitating antibody. (Ref.1)
Formulation	100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added. State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD3 antigen, epsilon polypeptide
Database Link	Entrez Gene 12501 Mouse UniProt ID P22646
Background	CD3e, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. (Ref.1,2) CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation.
Synonyms	T3/Leu-4
Reference Data

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