TPOR (MPL) Mouse Monoclonal Antibody [Clone ID: 1H2]

Specifications

Product Data
Clone Name	1H2
Applications	ELISA, FC, WB
Recommend Dilution	ELISA: 1/10000. Western Blot: 1/500 - 1/2000. Flow Cytometry: 1/200 - 1/400.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of Human MPL expressed in E. Coli.
Specificity	Recognizes MPL/CD110
Formulation	PBS State: Purified State: Liquid purified antibody Stabilizer: 0.5% protein stabilizer Preservative: 0.05% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	71.2 kDa
Gene Name	MPL proto-oncogene, thrombopoietin receptor
Database Link	Entrez Gene 4352 Human UniProt ID P40238
Background	In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated.
Synonyms	MPL, TPOR, TPO-R
Reference Data

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