Alkaline Phosphatase (ALPL) Mouse Monoclonal Antibody [Clone ID: 2F4]

Specifications

Product Data
Clone Name	2F4
Applications	ELISA, FC, IHC, WB
Recommend Dilution	ELISA: 1/10000. Western Blot: 1/500-1/2000. Flow Cytometry: 1/200-1/400. Immunohistochemistry on Paraffin Sections: 1/200-1/1000.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of Human ALPL expressed in E. Coli.
Specificity	Recognizes Human Alkaline phosphatase / ALPL. Other species not tested.
Formulation	PBS State: Purified State: Liquid purified antibody Stabilizer: 0.5% protein stabilizer Preservative: 0.05% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	57.3 kDa
Gene Name	alkaline phosphatase, liver/bone/kidney
Database Link	Entrez Gene 249 Human UniProt ID P05186
Background	There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants have been described.
Synonyms	AP-TNAP, TNSALP
Reference Data

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